Elution Profiles of Antibody-Drug Conjugates in Preparative Chromatography
نویسندگان
چکیده
Monoclonal antibody drug conjugate (ADCs) have received much attention as pharmaceutical agents for treating serious diseases such cancer. However, it is difficult to separate them on the basis of ratio, DAR. Hydrophobic chromatography (HIC) commonly used analysis The retention ADCs HIC can be controlled by hydrophobic nature ADCs, depending mobile phase conditions. They are sometimes performed at restricted conditions where solubility too low. Ion exchange (IEC) using electrostatic interaction an orthogonal method HIC. IEC widely because its higher capacity than We investigated behavior protein conjugated with surrogate drugs IEC. employed 7-diethylamino-3-(4’-maleimidylhenyl) 4-methylcoumarin (CPM), N-(1-pyrenyl) maleimide (NPM). Bovine serum albumin (BSA) was a model protein. molar ratio (CPM and NPM protein) set 3. group CPM reacts thiol proteins. On linear gradient elution experiments, salt concentrations non-conjugated proteins were measured obtain chromatographic parameter number binding sites, B .
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ژورنال
عنوان ژورنال: MATEC web of conferences
سال: 2021
ISSN: ['2261-236X', '2274-7214']
DOI: https://doi.org/10.1051/matecconf/202133314001